Hotspot

ndhD

SSC · 118884-120386 · 1503 bp

Back to Chrysanthemum

Pi 0.0017

MarkerSeek score 39.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 11
pi: 0.0017
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGGCACATGTTTTTCTGGT	CGGTTCTCGCAAAGACCAA	45.0	52.6	58.2	58.4	1656 bp	0.7	55.7
ndhD_p2	TGGGCACATGTTTTTCTGGT	GCAAAGACCAAGCGGATTG	45.0	52.6	58.2	57.6	1648 bp	0.7	55.6
ndhD_p3	TGTTTTTCTGGTCCAAGCGT	CGGTTCTCGCAAAGACCAA	45.0	52.6	58.2	58.4	1648 bp	0.7	55.5
ndhD_p4	TGTTTTTCTGGTCCAAGCGT	GCAAAGACCAAGCGGATTG	45.0	52.6	58.2	57.6	1640 bp	0.7	55.3
ndhD_p5	TGGGCACATGTTTTTCTGGT	CGGTTCTCGCAAAGACCAAG	45.0	55.0	58.2	59.5	1656 bp	0.7	54.9