Hotspot

cplP

LSC · 68704-70699 · 1996 bp

Back to Chrysanthemum

Pi 0.00166

MarkerSeek score 56.20

Primer available yes

Label cplP

Feature Metadata

label_name: cplP
feature_type: gene
region: LSC
length_bp: 1996
variable_sites: 13
pi: 0.00166
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cplP_p1	AAACTCGGCAGGGTGAATTT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2110-2148 bp	1.0	71.8
cplP_p2	AACTCGGCAGGGTGAATTTT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2109-2147 bp	1.0	71.8
cplP_p3	AAACTCGGCAGGGTGAATTT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2111-2149 bp	1.0	70.1
cplP_p4	AACTCGGCAGGGTGAATTTT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2110-2148 bp	1.0	70.1
cplP_p5	AACTCGGCAGGGTGAATTTT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	2110-2148 bp	1.0	69.4