Hotspot

trnL-UAG-rpl32

SSC · 121749-122623 · 875 bp

Back to Chrysanthemum

Pi 0.005

MarkerSeek score 61.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 875
variable_sites: 15
pi: 0.005
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGCGGCATTAAAAGCTTTGT	50.0	45.0	58.0	57.9	974-1001 bp	1.0	82.6
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGCGGCATTAAAAGCTTTGT	50.0	45.0	58.0	57.9	975-1002 bp	1.0	82.6
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCGGCATT	50.0	45.0	58.0	57.9	985-1012 bp	1.0	82.5
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCGGCATT	50.0	45.0	58.0	57.9	986-1013 bp	1.0	82.5
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GGCGGCATTAAAAGCTTTGT	50.0	45.0	57.9	57.9	984-1011 bp	1.0	81.7