Hotspot

ndhC-trnV-UAC

LSC · 49911-51057 · 1147 bp

Back to Chrysanthemum

Pi 0.00329

MarkerSeek score 58.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 12
pi: 0.00329
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGATCC	50.0	55.0	57.9	57.5	1290-1300 bp	1.0	76.0
ndhC-trnV-UAC_p2	ACTCGGAGCTAAAAATCCCG	GAAGGTCTACGGTTCGATCC	50.0	55.0	57.7	57.5	1281-1291 bp	1.0	74.7
ndhC-trnV-UAC_p3	TTCTCCGGTCCTTTGCTACT	GAAGGTCTACGGTTCGATCC	50.0	55.0	58.4	57.5	1298-1308 bp	1.0	74.3
ndhC-trnV-UAC_p4	TCCTTTGCTACTCGGAGCTA	GTTCGATCCCGTATAGCCCT	50.0	55.0	57.9	58.7	1279-1289 bp	1.0	74.0
ndhC-trnV-UAC_p5	TTTCTCCGGTCCTTTGCTAC	GAAGGTCTACGGTTCGATCC	50.0	55.0	57.5	57.5	1299-1309 bp	1.0	73.4