Hotspot

psaA-ycf3

LSC · 41149-41885 · 737 bp

Back to Chrysanthemum

Pi 0.00427

MarkerSeek score 65.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 12
pi: 0.00427
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	916-931 bp	1.0	78.5
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	918-933 bp	1.0	78.5
psaA-ycf3_p3	GGGCCTTTCGCTATTGTTCT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.2	58.3	937-952 bp	1.0	78.3
psaA-ycf3_p4	GGGCCTTTCGCTATTGTTCT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.2	58.3	939-954 bp	1.0	78.3
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	917-932 bp	1.0	76.7