Hotspot

trnS-GCU-trnC-GCA

LSC · 8536-9266 · 731 bp

Back to Chrysanthemum

Pi 0.00505
MarkerSeek score 52.70
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
731
variable_sites
12
pi
0.00505
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 882-903 bp 1.0 79.2
trnS-GCU-trnC-GCA_p2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 864-885 bp 1.0 77.0
trnS-GCU-trnC-GCA_p3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 818-839 bp 1.0 75.4
trnS-GCU-trnC-GCA_p4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 883-904 bp 1.0 75.0
trnS-GCU-trnC-GCA_p5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 800-821 bp 1.0 73.3