Hotspot

ycf36

Genome · 88315-88809 · 495 bp

Back to Chroomonas

Pi 0.376

MarkerSeek score 81.30

Primer available yes

Label ycf36

Feature Metadata

label_name: ycf36
feature_type: gene
region: Genome
length_bp: 495
variable_sites: 177
pi: 0.376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf36_p1	CAAGGGATGGGTGGACAAAA	CTCGTAGCCATCCTCCTGTA	50.0	55.0	58.0	58.0	973 bp	0.5	59.8
ycf36_p2	CAAGGGATGGGTGGACAAAA	ATGCATTAGACCAGCTCTGC	50.0	50.0	58.0	58.0	1410 bp	0.5	59.7
ycf36_p3	GTGCTGCAATCGTTTGTACC	CTCGTAGCCATCCTCCTGTA	50.0	55.0	58.0	58.0	1221 bp	0.5	59.6
ycf36_p4	CAAGGGATGGGTGGACAAAA	CATTGGCCTTCGGGTTATGA	50.0	50.0	58.0	57.9	807 bp	0.5	59.5
ycf36_p5	CAAGGGATGGGTGGACAAAA	AAGCATTGTTCTTTGCGAGC	50.0	45.0	58.0	57.9	1109 bp	0.5	59.5