Hotspot

ycf26

Genome · 38583-39281 · 699 bp

Back to Chroomonas

Pi 0.385

MarkerSeek score 83.30

Primer available yes

Label ycf26

Feature Metadata

label_name: ycf26
feature_type: gene
region: Genome
length_bp: 699
variable_sites: 257
pi: 0.385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf26_p1	CTCATGCGGCTTATGAAGGT	CAAGTCCCTTCACCAGCTTT	50.0	50.0	58.0	58.0	1460 bp	0.5	59.6
ycf26_p2	TTTGAACCAGTGACCACCTG	CAAGTCCCTTCACCAGCTTT	50.0	50.0	57.9	58.0	1080 bp	0.5	59.5
ycf26_p3	TTGTTACTAACCGAGCTGCC	CAAGTCCCTTCACCAGCTTT	50.0	50.0	57.9	58.0	1484 bp	0.5	59.2
ycf26_p4	AAGGTCTCTGTCCTGATCCC	CAAGTCCCTTCACCAGCTTT	55.0	50.0	58.1	58.0	1190 bp	0.5	58.9
ycf26_p5	GGTCTCTGTCCTGATCCCTT	CAAGTCCCTTCACCAGCTTT	55.0	50.0	58.1	58.0	1188 bp	0.5	58.9