Hotspot

rne

Genome · 46652-47965 · 1314 bp

Back to Chroomonas

Pi 0.383

MarkerSeek score 81.90

Primer available yes

Label rne

Feature Metadata

label_name: rne
feature_type: gene
region: Genome
length_bp: 1314
variable_sites: 487
pi: 0.383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rne_p1	TTATGAGTCCGTTGCCTTCG	AACAAACCCGAATCTTGGCT	50.0	45.0	58.0	58.0	2333 bp	0.5	59.9
rne_p2	TTATGAGTCCGTTGCCTTCG	ACAAACCCGAATCTTGGCTT	50.0	45.0	58.0	58.0	2332 bp	0.5	59.9
rne_p3	TTATGAGTCCGTTGCCTTCG	TCTGAAAAATGGGGTGGTGG	50.0	50.0	58.0	58.0	2095 bp	0.5	59.9
rne_p4	CTCGCCTTTCAGAATCGGAA	AACAAACCCGAATCTTGGCT	50.0	45.0	58.0	58.0	1825 bp	0.5	59.9
rne_p5	CTCGCCTTTCAGAATCGGAA	ACAAACCCGAATCTTGGCTT	50.0	45.0	58.0	58.0	1824 bp	0.5	59.9