Hotspot

dnaB

Genome · 130136-131941 · 1806 bp

Back to Chroomonas

Pi 0.352

MarkerSeek score 80.80

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1806
variable_sites: 619
pi: 0.352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTTGGTAGAGCAGTGGA	ACTCCCGCTGCTTTAGAAAG	50.0	50.0	58.0	57.9	2611 bp	0.5	59.1
dnaB_p2	TCAGTTGGTAGAGCAGTGGA	CTCCCGCTGCTTTAGAAAGT	50.0	50.0	58.0	57.9	2610 bp	0.5	59.1
dnaB_p3	GAGGGAGATACAGCGGTAGT	ACTCCCGCTGCTTTAGAAAG	55.0	50.0	58.0	57.9	2858 bp	0.5	59.1
dnaB_p4	GAGGGAGATACAGCGGTAGT	CTCCCGCTGCTTTAGAAAGT	55.0	50.0	58.0	57.9	2857 bp	0.5	59.1
dnaB_p5	TCAGTTGGTAGAGCAGTGGA	GCCTTAAACCACTCGACCAT	50.0	50.0	58.0	57.9	2045 bp	0.5	59.0