Hotspot

psbN-psbH

Genome · 120050-121056 · 1007 bp

Back to Chroomonas

Pi 0.404

MarkerSeek score 80.80

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: Genome
length_bp: 1007
variable_sites: 334
pi: 0.404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GGGACCAAAAGCTGTGTACA	GCTGCCTAAACTAGCCCAAT	50.0	50.0	58.0	57.9	1295 bp	0.5	59.3
psbN-psbH_p2	ATTACCCCTCATCCTACCCG	GCTGCCTAAACTAGCCCAAT	55.0	50.0	58.0	57.9	1885 bp	0.5	59.3
psbN-psbH_p3	TCAGATCTTTCGATCCGGGA	GCTGCCTAAACTAGCCCAAT	50.0	50.0	57.9	57.9	1311 bp	0.5	59.0
psbN-psbH_p4	GGGACCAAAAGCTGTGTACA	TAGCTGCCTAAACTAGCCCA	50.0	50.0	58.0	58.1	1297 bp	0.5	58.8
psbN-psbH_p5	ATTACCCCTCATCCTACCCG	TAGCTGCCTAAACTAGCCCA	55.0	50.0	58.0	58.1	1887 bp	0.5	58.8