Hotspot

psbN-psbN

Genome · 117662-119925 · 2264 bp

Back to Chroomonas

Pi 0.393

MarkerSeek score 78.00

Primer available yes

Label psbN-psbN

Feature Metadata

label_name: psbN-psbN
feature_type: igs
region: Genome
length_bp: 2264
variable_sites: 702
pi: 0.393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbN_p1	AGCCCTAGAGGATGGTACAC	TGTACACAGCTTTTGGTCCC	55.0	50.0	57.9	58.0	2758 bp	0.5	59.1
psbN-psbN_p2	GGAGGAAAACTAAACGGGCA	TGTACACAGCTTTTGGTCCC	50.0	50.0	58.1	58.0	2887 bp	0.5	59.0
psbN-psbN_p3	GCAGAAGTGACTGAGCAAGT	TGTACACAGCTTTTGGTCCC	50.0	50.0	58.1	58.0	2644 bp	0.5	58.8
psbN-psbN_p4	AGCCCTAGAGGATGGTACAC	TAGGTCCCTTCCGCTTTACA	55.0	50.0	57.9	58.1	2901 bp	0.5	58.8
psbN-psbN_p5	TGCAGAAGTGACTGAGCAAG	TGTACACAGCTTTTGGTCCC	50.0	50.0	58.1	58.0	2645 bp	0.5	58.8