Hotspot

trnK-UUU-ycf12

Genome · 51234-51787 · 554 bp

Back to Chroomonas

Pi 0.374

MarkerSeek score 81.30

Primer available yes

Label trnK-UUU-ycf12

Feature Metadata

label_name: trnK-UUU-ycf12
feature_type: igs
region: Genome
length_bp: 554
variable_sites: 179
pi: 0.374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-ycf12_p1	AAGGTAAGTACAGCCCCCAT	TATGGAGCTCCTCTATGCCC	50.0	55.0	58.0	58.1	1248 bp	0.5	59.4
trnK-UUU-ycf12_p2	AAGGTAAGTACAGCCCCCAT	TAGCGCGACACAAAGATGAA	50.0	45.0	58.0	57.9	1268 bp	0.5	59.3
trnK-UUU-ycf12_p3	AAGGTAAGTACAGCCCCCAT	TTAGCGCGACACAAAGATGA	50.0	45.0	58.0	57.9	1269 bp	0.5	59.3
trnK-UUU-ycf12_p4	TCACGGAGGTAACAGGGATT	TATGGAGCTCCTCTATGCCC	50.0	55.0	58.1	58.1	1201 bp	0.5	59.1
trnK-UUU-ycf12_p5	TTCACGGAGGTAACAGGGAT	TATGGAGCTCCTCTATGCCC	50.0	55.0	58.1	58.1	1202 bp	0.5	59.1