Hotspot

rnl-trnA-UGC

Genome · 34567-34638 · 72 bp

Back to Chroomonas

Pi 0.143

MarkerSeek score 80.70

Primer available yes

Label rnl-trnA-UGC

Feature Metadata

label_name: rnl-trnA-UGC
feature_type: igs
region: Genome
length_bp: 72
variable_sites: 10
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnl-trnA-UGC_p1	TCATCGCTTCTGAATGCCAA	GGGGGTATAGCTCAGTTGGT	45.0	55.0	57.9	58.5	197-200 bp	1.0	80.0
rnl-trnA-UGC_p2	TTCATCGCTTCTGAATGCCA	GGGGGTATAGCTCAGTTGGT	45.0	55.0	57.9	58.5	198-201 bp	1.0	80.0
rnl-trnA-UGC_p3	CGCTTCTGAATGCCAAGGTA	GGGGGTATAGCTCAGTTGGT	50.0	55.0	58.3	58.5	193-196 bp	1.0	78.9
rnl-trnA-UGC_p4	GCCCTTCATCGCTTCTGAAT	GGGGGTATAGCTCAGTTGGT	50.0	55.0	58.3	58.5	202-205 bp	1.0	78.2
rnl-trnA-UGC_p5	AATGCTTACTTCCAGCTCCC	GGGGGTATAGCTCAGTTGGT	50.0	55.0	57.6	58.5	245-248 bp	1.0	76.5