Hotspot

trnA-UGC-rnl

Genome · 9501-9572 · 72 bp

Back to Chroomonas

Pi 0.143

MarkerSeek score 80.70

Primer available yes

Label trnA-UGC-rnl

Feature Metadata

label_name: trnA-UGC-rnl
feature_type: igs
region: Genome
length_bp: 72
variable_sites: 10
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-rnl_p1	GGGGGTATAGCTCAGTTGGT	TCATCGCTTCTGAATGCCAA	55.0	45.0	58.5	57.9	197-200 bp	1.0	80.0
trnA-UGC-rnl_p2	GGGGGTATAGCTCAGTTGGT	TTCATCGCTTCTGAATGCCA	55.0	45.0	58.5	57.9	198-201 bp	1.0	80.0
trnA-UGC-rnl_p3	GGGGGTATAGCTCAGTTGGT	CGCTTCTGAATGCCAAGGTA	55.0	50.0	58.5	58.3	193-196 bp	1.0	78.9
trnA-UGC-rnl_p4	GGGGGTATAGCTCAGTTGGT	GCCCTTCATCGCTTCTGAAT	55.0	50.0	58.5	58.3	202-205 bp	1.0	78.2
trnA-UGC-rnl_p5	GGGGGTATAGCTCAGTTGGT	AATGCTTACTTCCAGCTCCC	55.0	50.0	58.5	57.6	245-248 bp	1.0	76.5