Hotspot

ycf1-chlN

SSC · 124146-125640 · 1495 bp

Back to Christella

Pi 0.0156

MarkerSeek score 54.90

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 1495
variable_sites: 23
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	CTTTCGCCGACAAAGTTTCC	GGGAGATGAAGAAGCCCATG	50.0	55.0	58.0	58.0	2113-2131 bp	1.0	81.5
ycf1-chlN_p2	CAACAACTTGACTCGACCCA	GGGAGATGAAGAAGCCCATG	50.0	55.0	58.1	58.0	1788-1806 bp	1.0	81.5
ycf1-chlN_p3	AAAAATGTTTGTGGCACCGG	GGGAGATGAAGAAGCCCATG	45.0	55.0	58.1	58.0	1925-1943 bp	1.0	81.4
ycf1-chlN_p4	CTTTCGCCGACAAAGTTTCC	ACTCTATTTTAGGCTGCCGC	50.0	50.0	58.0	58.0	1873-1891 bp	1.0	81.4
ycf1-chlN_p5	CTTTCGCCGACAAAGTTTCC	GAACAGCGACAACACCAATG	50.0	50.0	58.0	58.0	2455-2473 bp	1.0	81.2