Hotspot

psaC-ndhE

SSC · 114061-114371 · 311 bp

Back to Christella

Pi 0.0225

MarkerSeek score 64.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 311
variable_sites: 7
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCCATCCCAGGGTATCATT	CACTGCCCTTTCTCTCCATC	50.0	55.0	58.2	58.0	481-482 bp	1.0	79.6
psaC-ndhE_p2	ATGCCCTTACACACTGGGTA	CACTGCCCTTTCTCTCCATC	50.0	55.0	58.3	58.0	442-443 bp	1.0	78.3
psaC-ndhE_p3	AGCCATCCCAGGGTATCATT	TCAACATTTGTGACAGCCGT	50.0	45.0	58.2	58.3	531-532 bp	1.0	77.2
psaC-ndhE_p4	AGCCATCCCAGGGTATCATT	GCCCTTTCTCTCCATCGAAA	50.0	50.0	58.2	57.6	477-478 bp	1.0	76.7
psaC-ndhE_p5	GACATGCCCTTACACACTGG	CACTGCCCTTTCTCTCCATC	55.0	55.0	58.5	58.0	445-446 bp	1.0	76.6