Hotspot

trnP-GGG-trnL-UAG

SSC · 110176-110433 · 258 bp

Back to Christella

Pi 0.0388
MarkerSeek score 65.40
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
SSC
length_bp
258
variable_sites
10
pi
0.0388
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GATTCGAACCTGTGACCTCC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 373-403 bp 1.0 81.8
trnP-GGG-trnL-UAG_p2 GATTCGAACCTGTGACCTCC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 371-401 bp 1.0 81.8
trnP-GGG-trnL-UAG_p3 GGATTCGAACCTGTGACCTC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 374-404 bp 1.0 81.8
trnP-GGG-trnL-UAG_p4 GGATTCGAACCTGTGACCTC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 372-402 bp 1.0 81.8
trnP-GGG-trnL-UAG_p5 GATTCGAACCTGTGACCTCC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 360-390 bp 1.0 81.0