Hotspot

ycf4-cemA

LSC · 57005-57187 · 183 bp

Back to Christella

Pi 0.0383

MarkerSeek score 64.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 7
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCCGCGTGTATCGATTGAAG	GCCTCATAAGCTCTATCCGG	50.0	55.0	58.2	57.3	294 bp	1.0	75.3
ycf4-cemA_p2	TCCGCGTGTATCGATTGAAG	TAAGCTCTATCCGGGGAACG	50.0	55.0	58.2	58.7	288 bp	1.0	75.2
ycf4-cemA_p3	TTCCGCGTGTATCGATTGAA	GCCTCATAAGCTCTATCCGG	45.0	55.0	57.7	57.3	295 bp	1.0	74.3
ycf4-cemA_p4	TTTCCGCGTGTATCGATTGA	GCCTCATAAGCTCTATCCGG	45.0	55.0	57.7	57.3	296 bp	1.0	74.3
ycf4-cemA_p5	TTCCGCGTGTATCGATTGAA	TAAGCTCTATCCGGGGAACG	45.0	55.0	57.7	58.7	289 bp	1.0	74.3