Hotspot

psbM-petN

LSC · 28334-29204 · 871 bp

Back to Christella

Pi 0.0161

MarkerSeek score 68.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 14
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCCACCGCACTTTTTATTCC	TCTCACTTGTAGTTCGGGGA	50.0	50.0	58.0	57.7	992-993 bp	1.0	78.7
psbM-petN_p2	GCCACCGCACTTTTTATTCC	TTGTAGTTCGGGGAAGAAGC	50.0	50.0	58.0	57.5	986-987 bp	1.0	77.3
psbM-petN_p3	GCCACCGCACTTTTTATTCC	CCCTCTCACTTGTAGTTCGG	50.0	55.0	58.0	57.3	995-996 bp	1.0	75.8
psbM-petN_p4	GCCACCGCACTTTTTATTCC	CCTCTCACTTGTAGTTCGGG	50.0	55.0	58.0	57.3	994-995 bp	1.0	75.8
psbM-petN_p5	GCCACCGCACTTTTTATTCC	CTCTCACTTGTAGTTCGGGG	50.0	55.0	58.0	57.3	993-994 bp	1.0	75.8