Hotspot

trnD-GUC-trnY-GUA

LSC · 27431-27487 · 57 bp

Back to Christella

Pi 0.0526

MarkerSeek score 73.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 3
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	AATTGGTTAGAGCACCGACC	GATGCTCGAGTGGCTAATGG	50.0	55.0	57.9	58.5	195-206 bp	1.0	76.4
trnD-GUC-trnY-GUA_p2	AATTGGTTAGAGCACCGACC	GACTGTAAATCCGCTGGCAA	50.0	50.0	57.9	58.6	170-181 bp	1.0	76.1
trnD-GUC-trnY-GUA_p3	ACCTGTCAAGTCGGAAGTTG	GATGCTCGAGTGGCTAATGG	50.0	55.0	57.8	58.5	178-189 bp	1.0	75.4
trnD-GUC-trnY-GUA_p4	ACCTGTCAAGTCGGAAGTTG	GACTGTAAATCCGCTGGCAA	50.0	50.0	57.8	58.6	153-164 bp	1.0	75.2
trnD-GUC-trnY-GUA_p5	AATTGGTTAGAGCACCGACC	ACTGTAAATCCGCTGGCAAC	50.0	50.0	57.9	58.8	169-180 bp	1.0	73.8