Hotspot

rpoC2-trnD-GUC

LSC · 20047-27356 · 7310 bp

Back to Christella

Pi 0.00725
MarkerSeek score 48.20
Primer available yes
Label rpoC2-trnD-GUC

Feature Metadata

label_name
rpoC2-trnD-GUC
feature_type
igs
region
LSC
length_bp
7310
variable_sites
53
pi
0.00725
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-trnD-GUC_p1 GCTACAAAAAGAAGTTACTCGGT ACCCTGAGTCAGCAATCTCT 39.1 50.0 57.1 58.1 188 bp 1.0 51.6
rpoC2-trnD-GUC_p2 GCTACAAAAAGAAGTTACTCGGT GTCAACCCTGAGTCAGCAAT 39.1 50.0 57.1 57.8 192 bp 1.0 50.5
rpoC2-trnD-GUC_p3 AGCTACAAAAAGAAGTTACTCGG ACCCTGAGTCAGCAATCTCT 39.1 50.0 56.8 58.1 189 bp 1.0 49.6
rpoC2-trnD-GUC_p4 AGCTACAAAAAGAAGTTACTCGG GTCAACCCTGAGTCAGCAAT 39.1 50.0 56.8 57.8 193 bp 1.0 48.4
rpoC2-trnD-GUC_p5 GCTACAAAAAGAAGTTACTCGGT TCAACCCTGAGTCAGCAATC 39.1 50.0 57.1 57.5 191 bp 1.0 48.2