Hotspot

atpH-atpI

LSC · 13566-14319 · 754 bp

Back to Christella

Pi 0.012

MarkerSeek score 65.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 9
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTAACCCAGCAGCAATGACA	AGCTCATATCGGGGAATCCA	50.0	50.0	58.2	58.0	826-828 bp	1.0	79.4
atpH-atpI_p2	TAACCCAGCAGCAATGACAG	AGCTCATATCGGGGAATCCA	50.0	50.0	58.2	58.0	825-827 bp	1.0	79.4
atpH-atpI_p3	CTAACCCAGCAGCAATGACA	GTACCCACGATGCCTTTAGG	50.0	55.0	58.2	58.1	894-896 bp	1.0	79.2
atpH-atpI_p4	TAACCCAGCAGCAATGACAG	GTACCCACGATGCCTTTAGG	50.0	55.0	58.2	58.1	893-895 bp	1.0	79.2
atpH-atpI_p5	TAACCCAGCAGCAATGACAG	GCTCATATCGGGGAATCCAC	50.0	55.0	58.2	57.9	824-826 bp	1.0	78.9