Hotspot

rpl2

LSC · 85172-86690 · 1519 bp

Back to Chorispora

Pi 0.000658

MarkerSeek score 59.10

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1519
variable_sites: 1
pi: 0.000658
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	ACGTGTCACAGTTAATTAACTCCT	AAGGGGTCTTCAAAAGCGTT	37.5	45.0	58.1	57.9	1686 bp	1.0	46.3
rpl2_p2	ACGTGTCACAGTTAATTAACTCC	AAGGGGTCTTCAAAAGCGTT	39.1	45.0	56.8	57.9	1686 bp	1.0	46.2
rpl2_p3	AATGCTTTGCTACAAAAGGA	AAGGGGTCTTCAAAAGCGTT	35.0	45.0	53.7	57.9	1720 bp	1.0	44.8
rpl2_p4	TGCTTTGCTACAAAAGGATT	AAGGGGTCTTCAAAAGCGTT	35.0	45.0	53.7	57.9	1718 bp	1.0	44.8
rpl2_p5	ACGTGTCACAGTTAATTAACTCCT	CGCTTCAACCGGGTTATTCT	37.5	50.0	58.1	58.3	1648 bp	1.0	44.7