Hotspot

matK

LSC · 1978-3492 · 1515 bp

Back to Chorispora

Pi 0.00066

MarkerSeek score 59.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 1
pi: 0.00066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGCTAGGTCTCATAACCCAA	GACTGTATCGCACTATGTGTCA	45.0	45.5	55.5	58.0	1612 bp	1.0	44.1
matK_p2	TGCTAGGTCTCATAACCCAA	TGACTGTATCGCACTATGTGTC	45.0	45.5	55.5	58.0	1613 bp	1.0	44.1
matK_p3	TGCTAGGTCTCATAACCCAA	ACCTCATTTTGACTGTATCGCA	45.0	40.9	55.5	58.1	1622 bp	1.0	43.8
matK_p4	GGTATGCTAGGTCTCATAACCC	GACTGTATCGCACTATGTGTCA	50.0	45.5	57.5	58.0	1616 bp	1.0	43.5
matK_p5	GGTATGCTAGGTCTCATAACCC	TGACTGTATCGCACTATGTGTC	50.0	45.5	57.5	58.0	1617 bp	1.0	43.5