Hotspot

ycf1-trnN-AAC

IRa · 138541-139710 · 1170 bp

Back to Chorispora

Pi 0.00108

MarkerSeek score 59.60

Primer available yes

Label ycf1-trnN-AAC

Feature Metadata

label_name: ycf1-trnN-AAC
feature_type: igs
region: IRa
length_bp: 1170
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-AAC_p1	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.2	57.7	1076-1324 bp	1.0	80.1
ycf1-trnN-AAC_p2	ACCACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.8	57.7	1066-1314 bp	1.0	80.0
ycf1-trnN-AAC_p3	GGGACCTATGGATAATGCGG	CCCCAAGTAGGATTCGAACC	55.0	55.0	57.6	57.7	1109-1357 bp	1.0	78.2
ycf1-trnN-AAC_p4	GACCACAACGACCGAATTGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.5	57.7	1067-1315 bp	1.0	77.5
ycf1-trnN-AAC_p5	AATAGAGTCCGACCACAACG	CCCCAAGTAGGATTCGAACC	50.0	55.0	57.4	57.7	1077-1325 bp	1.0	76.3