Hotspot

petA-psbJ

LSC · 63345-64182 · 838 bp

Back to Chorispora

Pi 0.00119

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 1
pi: 0.00119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1025 bp	1.0	75.7
petA-psbJ_p2	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.4	58.1	999 bp	1.0	74.5
petA-psbJ_p3	TCCATTACGTGTCCAAGGAC	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.2	58.1	1031 bp	1.0	73.0
petA-psbJ_p4	CCATTACGTGTCCAAGGACT	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.2	58.1	1030 bp	1.0	72.9
petA-psbJ_p5	AGGATCGGTTGTTTTGGCAC	GCTGGTATTCCTGTGATCGG	50.0	55.0	59.0	58.1	998 bp	1.0	70.7