Hotspot

rbcL-psaI

LSC · 55066-59902 · 4837 bp

Back to Chorispora

Pi 0.00935

MarkerSeek score 62.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 4837
variable_sites: 43
pi: 0.00935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GCAATTAAACCCGGCTCAAC	CTTGATAGCCATTCCCACCC	50.0	55.0	58.0	58.0	2361-2368 bp	1.0	84.5
rbcL-psaI_p2	GCAATTAAACCCGGCTCAAC	TAGCCATTCCCAATGTTCCG	50.0	50.0	58.0	57.9	2443-2450 bp	1.0	84.0
rbcL-psaI_p3	GCAATTAAACCCGGCTCAAC	TTTCCGACGAATCCGAATCC	50.0	50.0	58.0	58.1	2483-2490 bp	1.0	83.8
rbcL-psaI_p4	ACCTCGACCTCTTTACGGAT	TCCGGCTAGAGGTAGTTACC	50.0	55.0	57.9	57.6	342-386 bp	1.0	82.2
rbcL-psaI_p5	GCAATTAAACCCGGCTCAAC	GCTGTTCAAATTCGGCTTCC	50.0	50.0	58.0	58.0	2393 bp	0.5	59.8