Hotspot

trnS-AGC-trnG-GGA

LSC · 7629-8245 · 617 bp

Back to Chorispora

Pi 0.00324

MarkerSeek score 60.60

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 2
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCACTCAGCCATCTCTCCTA	TGAATCAAACCGAGAGACCC	55.0	50.0	57.9	57.2	720-738 bp	1.0	73.5
trnS-AGC-trnG-GGA_p2	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	733-751 bp	1.0	72.8
trnS-AGC-trnG-GGA_p3	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	731-749 bp	1.0	72.5
trnS-AGC-trnG-GGA_p4	CCACTCAGCCATCTCTCCTA	GAATCAAACCGAGAGACCCT	55.0	50.0	57.9	56.9	719-737 bp	1.0	71.1
trnS-AGC-trnG-GGA_p5	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	728-746 bp	1.0	70.8