Hotspot

trnK-AAA-rps16

LSC · 4260-4946 · 687 bp

Back to Chorispora

Pi 0

MarkerSeek score 61.70

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAGGCGGGGATTTTTACGT	50.0	45.0	58.2	58.1	752-773 bp	1.0	80.2
trnK-AAA-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAGGCGGGGATTTTTACGT	50.0	45.0	58.2	58.1	751-772 bp	1.0	80.2
trnK-AAA-rps16_p3	TCTACCGCATATCTGGACGA	AAAGGCGGGGATTTTTACGT	50.0	45.0	57.7	58.1	817-838 bp	1.0	79.2
trnK-AAA-rps16_p4	CCGCATATCTGGACGAATCC	AAAGGCGGGGATTTTTACGT	55.0	45.0	58.3	58.1	813-834 bp	1.0	79.1
trnK-AAA-rps16_p5	ACCGCATATCTGGACGAATC	AAAGGCGGGGATTTTTACGT	50.0	45.0	57.6	58.1	814-835 bp	1.0	77.8