Hotspot

ndhD

SSC · 117286-118761 · 1476 bp

Back to Chorilaena

Pi 0.0061

MarkerSeek score 51.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1476
variable_sites: 9
pi: 0.0061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGTTCATTGGCTACCGAACT	GGGCCTAGTGTATCTTGTCT	45.0	50.0	57.1	56.0	1600-1601 bp	1.0	57.9
ndhD_p2	TGTTCATTGGCTACCGAACT	GGGCCTAGTGTATCTTGTCTT	45.0	47.6	57.1	56.8	1600-1601 bp	1.0	56.1
ndhD_p3	TGTTCATTGGCTACCGAACTT	GGGCCTAGTGTATCTTGTCT	42.9	50.0	57.9	56.0	1600-1601 bp	1.0	55.6
ndhD_p4	TTGTTCATTGGCTACCGAACT	GGGCCTAGTGTATCTTGTCT	42.9	50.0	57.9	56.0	1601-1602 bp	1.0	55.6
ndhD_p5	TGTTCATTGGCTACCGAACTT	GGGCCTAGTGTATCTTGTCTT	42.9	47.6	57.9	56.8	1600-1601 bp	1.0	53.8