Hotspot

ccsA-ndhD

SSC · 116966-117285 · 320 bp

Back to Chorilaena

Pi 0.0635

MarkerSeek score 78.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 19
pi: 0.0635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAATTGTCGCTTCTACGGGA	AGTGGACAAGGTCGAAGCTA	50.0	50.0	58.0	58.4	455-461 bp	1.0	84.0
ccsA-ndhD_p2	CAATTCCGCAATTGTCGCTT	AGTGGACAAGGTCGAAGCTA	45.0	50.0	58.0	58.4	463-469 bp	1.0	83.7
ccsA-ndhD_p3	TGAAGGTGTCAATTCCGCAA	AGTGGACAAGGTCGAAGCTA	45.0	50.0	58.0	58.4	472-478 bp	1.0	83.6
ccsA-ndhD_p4	TTGAAGGTGTCAATTCCGCA	AGTGGACAAGGTCGAAGCTA	45.0	50.0	58.0	58.4	473-479 bp	1.0	83.5
ccsA-ndhD_p5	TTCCGCAATTGTCGCTTCTA	AGTGGACAAGGTCGAAGCTA	45.0	50.0	57.9	58.4	460-466 bp	1.0	83.2