Hotspot

trnW-CCA-trnP-UGG

LSC · 68108-68315 · 208 bp

Back to Chorilaena

Pi 0.0476

MarkerSeek score 69.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 6
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	205-287 bp	1.0	84.9
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	194-276 bp	1.0	84.9
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	192-274 bp	1.0	84.9
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	195-277 bp	1.0	84.9
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	206-288 bp	1.0	84.8