Hotspot

petN-psbM

LSC · 29592-30848 · 1257 bp

Back to Chorilaena

Pi 0.0176

MarkerSeek score 62.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1257
variable_sites: 22
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TCGTTCTAGTTCCTACTGCCT	45.0	47.6	58.1	57.9	1364-1367 bp	1.0	72.1
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1357-1360 bp	1.0	71.9
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1356-1359 bp	1.0	71.9
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1355-1358 bp	1.0	71.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TCGTTCTAGTTCCTACTGCCT	50.0	47.6	58.2	57.9	1402-1405 bp	1.0	71.7