Hotspot

atpH-atpI

LSC · 14172-15345 · 1174 bp

Back to Chorilaena

Pi 0.0225

MarkerSeek score 62.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 26
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1261-1271 bp	1.0	81.4
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1262-1272 bp	1.0	80.2
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	AGGCGAATCTATGGAGGGAC	45.0	55.0	58.0	58.7	1234-1244 bp	1.0	77.3
atpH-atpI_p4	TAACGGAAGCGGCAGAAATC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.6	57.9	1260-1270 bp	1.0	76.6
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.0	57.2	1284-1294 bp	1.0	76.3