Hotspot

trnR-UCU-atpA

LSC · 10288-10527 · 240 bp

Back to Chorilaena

Pi 0.0278

MarkerSeek score 63.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 6
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CTGAAAGGCGTCCATTGTCT	CGAGGAAGCAGAAACCCTTT	50.0	50.0	58.2	58.1	371-395 bp	1.0	82.9
trnR-UCU-atpA_p2	CTGAAAGGCGTCCATTGTCT	TTCACCGAGGAAGCAGAAAC	50.0	50.0	58.2	58.1	376-400 bp	1.0	82.8
trnR-UCU-atpA_p3	CTGAAAGGCGTCCATTGTCT	GGAAGCAGAAACCCTTTTGC	50.0	50.0	58.2	57.8	368-392 bp	1.0	82.5
trnR-UCU-atpA_p4	ACTGAAAGGCGTCCATTGTC	CGAGGAAGCAGAAACCCTTT	50.0	50.0	58.5	58.1	372-396 bp	1.0	80.6
trnR-UCU-atpA_p5	GACTGAAAGGCGTCCATTGT	CGAGGAAGCAGAAACCCTTT	50.0	50.0	58.5	58.1	373-397 bp	1.0	80.6