Hotspot

ycf39

Genome · 117980-118939 · 960 bp

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Pi 0.44
MarkerSeek score 80.50
Primer available yes
Label ycf39

Feature Metadata

label_name
ycf39
feature_type
gene
region
Genome
length_bp
960
variable_sites
415
pi
0.44
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf39_p1 AGGTTTACCTCTGGTTCCTCA GTATCGCCTCGTTCTTGGTT 47.6 50.0 58.0 58.0 2211 bp 0.5 51.8
ycf39_p2 AGGTTTACCTCTGGTTCCTCA GCTGTTCTTTCAATGCGTCC 47.6 50.0 58.0 58.0 2076 bp 0.5 51.6
ycf39_p3 AGGTTTACCTCTGGTTCCTCA TGGGATTTACGTGCTCCATG 47.6 50.0 58.0 58.0 2394 bp 0.5 51.6
ycf39_p4 AGGTTTACCTCTGGTTCCTCA GATTTACGTGCTCCATGGGT 47.6 50.0 58.0 58.0 2391 bp 0.5 51.6
ycf39_p5 GGTTTACCTCTGGTTCCTCAC GTATCGCCTCGTTCTTGGTT 52.4 50.0 57.9 58.0 2210 bp 0.5 51.5