Hotspot

ycf24

Genome · 122971-124416 · 1446 bp

Back to Chorda

Pi 0.439
MarkerSeek score 80.30
Primer available yes
Label ycf24

Feature Metadata

label_name
ycf24
feature_type
gene
region
Genome
length_bp
1446
variable_sites
625
pi
0.439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf24_p1 ATCCACTCCCGCAATTTCAA TCCTTTCGTGGAGCATCATT 45.0 45.0 57.8 57.2 2293 bp 0.5 52.0
ycf24_p2 CCCCCTGAAAACCCTTCATT TCCTTTCGTGGAGCATCATT 50.0 45.0 57.7 57.2 2457 bp 0.5 51.1
ycf24_p3 CACTCCCGCAATTTCAACTG TCCTTTCGTGGAGCATCATT 50.0 45.0 57.7 57.2 2290 bp 0.5 51.0
ycf24_p4 ATCCACTCCCGCAATTTCAA GGATATCCTTTCGTGGAGCA 45.0 50.0 57.8 57.1 2298 bp 0.5 51.0
ycf24_p5 ATCCACTCCCGCAATTTCAA TGGATATCCTTTCGTGGAGC 45.0 50.0 57.8 57.1 2299 bp 0.5 51.0