Hotspot

syfB

Genome · 126813-129113 · 2301 bp

Back to Chorda

Pi 0.461
MarkerSeek score 80.60
Primer available yes
Label syfB

Feature Metadata

label_name
syfB
feature_type
gene
region
Genome
length_bp
2301
variable_sites
1021
pi
0.461
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
syfB_p1 TGGAACAATGGACAATGGCA GAAAAACGCTGAAGCTTGGG 45.0 50.0 58.0 58.2 2534 bp 0.5 58.1
syfB_p2 TGGAACAATGGACAATGGCA AAAACGCTGAAGCTTGGGAT 45.0 45.0 58.0 58.4 2532 bp 0.5 56.9
syfB_p3 TGGAACAATGGACAATGGCA TGAAAAACGCTGAAGCTTGG 45.0 45.0 58.0 57.5 2535 bp 0.5 56.0
syfB_p4 TGGAACAATGGACAATGGCA ACCAAAAACGCTTCAAGCTC 45.0 45.0 58.0 57.5 2564 bp 0.5 56.0
syfB_p5 TGGAACAATGGACAATGGCA TGAAGCTTGGGATCGTTTCA 45.0 45.0 58.0 57.4 2525 bp 0.5 55.5