Hotspot

rpoC2

Genome · 39635-43480 · 3846 bp

Pi 0.435

MarkerSeek score 77.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 3846
variable_sites: 1517
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACCTTGAATAGCAGCTCGTG	CATTTTAGAAGCCCGCAAGC	50.0	50.0	58.0	58.1	631 bp	0.5	59.2
rpoC2_p2	CCTTGAATAGCAGCTCGTGT	CATTTTAGAAGCCCGCAAGC	50.0	50.0	58.0	58.1	630 bp	0.5	59.2
rpoC2_p3	GCTGCAGCTAAAAATCCGTC	CATTTTAGAAGCCCGCAAGC	50.0	50.0	57.8	58.1	582 bp	0.5	57.9
rpoC2_p4	TCTTGGAAACTAGCTGCAGC	CATTTTAGAAGCCCGCAAGC	50.0	50.0	58.2	58.1	594 bp	0.5	57.8
rpoC2_p5	CCAGCAGGGATTAGTCTTCC	CATTTTAGAAGCCCGCAAGC	55.0	50.0	57.4	58.1	693 bp	0.5	54.3