Hotspot

psbW

Genome · 129194-129538 · 345 bp

Back to Chorda

Pi 0.445
MarkerSeek score 75.70
Primer available yes
Label psbW

Feature Metadata

label_name
psbW
feature_type
gene
region
Genome
length_bp
345
variable_sites
150
pi
0.445
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbW_p1 GCCCTATTAGTTCCTGTACCC TGATTCTTTTTGTTTCAGCGCA 52.4 36.4 57.2 58.0 843 bp 0.5 29.6
psbW_p2 GCCCTATTAGTTCCTGTACCC TCGGATATACCCAGGTTTGAC 52.4 47.6 57.2 56.9 762 bp 0.5 29.0
psbW_p3 GCCCTATTAGTTCCTGTACCC CGGATATACCCAGGTTTGACT 52.4 47.6 57.2 56.9 761 bp 0.5 28.9
psbW_p4 GCCCTATTAGTTCCTGTACCC ACTTGTTGTTGCAATTTCAGCA 52.4 36.4 57.2 58.1 807 bp 0.5 28.7
psbW_p5 GCCCTATTAGTTCCTGTACCC TCGGATATACCCAGGTTTGACT 52.4 45.5 57.2 58.4 762 bp 0.5 27.1