Hotspot

psbD

Genome · 120714-121766 · 1053 bp

Back to Chorda

Pi 0.442
MarkerSeek score 81.30
Primer available yes
Label psbD

Feature Metadata

label_name
psbD
feature_type
gene
region
Genome
length_bp
1053
variable_sites
453
pi
0.442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD_p1 ATCTAACGTCTCCACCACCA AAATCCTACCTGGGGAGCTT 50.0 50.0 58.1 58.0 1850 bp 0.5 59.4
psbD_p2 ATCTAACGTCTCCACCACCA AATCCTACCTGGGGAGCTTT 50.0 50.0 58.1 58.0 1849 bp 0.5 59.4
psbD_p3 CAATCCAACCATCACCACCA AAATCCTACCTGGGGAGCTT 50.0 50.0 58.1 58.0 1931 bp 0.5 59.3
psbD_p4 CAATCCAACCATCACCACCA AATCCTACCTGGGGAGCTTT 50.0 50.0 58.1 58.0 1930 bp 0.5 59.3
psbD_p5 ATCTAACGTCTCCACCACCA TACCTGGGGAGCTTTCTTGA 50.0 50.0 58.1 58.0 1844 bp 0.5 59.2