Hotspot

cp74

Genome · 82800-84704 · 1905 bp

Pi 0.405

MarkerSeek score 82.00

Primer available yes

Label cp74

Feature Metadata

label_name: cp74
feature_type: gene
region: Genome
length_bp: 1905
variable_sites: 182
pi: 0.405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.24
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cp74_p1	GTTGCGATTTCAGCACCTTC	GGCAAAGAAGGTCTTCCACA	50.0	50.0	58.0	58.0	2912 bp	0.5	59.6
cp74_p2	GTTGCGATTTCAGCACCTTC	TAGTGGCTGGATAGGATGGG	50.0	55.0	58.0	58.0	2823 bp	0.5	59.5
cp74_p3	TTGCGATTTCAGCACCTTCT	GGCAAAGAAGGTCTTCCACA	45.0	50.0	58.1	58.0	2911 bp	0.5	59.0
cp74_p4	TTGCGATTTCAGCACCTTCT	TAGTGGCTGGATAGGATGGG	45.0	55.0	58.1	58.0	2822 bp	0.5	58.9
cp74_p5	GTTGCGATTTCAGCACCTTC	AGAAGGTCTTCCACACCTGA	50.0	50.0	58.0	57.9	2907 bp	0.5	58.9