Hotspot

atpI

Genome · 37252-37989 · 738 bp

Pi 0.341

MarkerSeek score 83.60

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 738
variable_sites: 239
pi: 0.341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GCAGCTAAACCAACAGCAAG	CGTCAAAAAGGCTTAGCAGC	50.0	50.0	57.9	58.0	1510 bp	0.5	59.3
atpI_p2	GCAGCTAAACCAACAGCAAG	GGCTTAGCAGCTGCAGATAA	50.0	50.0	57.9	58.0	1501 bp	0.5	59.2
atpI_p3	CAGCAGTTCCCTGTCCAATT	CGTCAAAAAGGCTTAGCAGC	50.0	50.0	58.1	58.0	1542 bp	0.5	59.1
atpI_p4	CAGCAGTTCCCTGTCCAATT	GGCTTAGCAGCTGCAGATAA	50.0	50.0	58.1	58.0	1533 bp	0.5	59.0
atpI_p5	AGCTAAGGCCACAACTAAGC	CGTCAAAAAGGCTTAGCAGC	50.0	50.0	57.9	58.0	1667 bp	0.5	58.8