Hotspot

atpD

Genome · 35143-35760 · 618 bp

Pi 0.457

MarkerSeek score 83.10

Primer available yes

Label atpD

Feature Metadata

label_name: atpD
feature_type: gene
region: Genome
length_bp: 618
variable_sites: 263
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpD_p1	ACTCACGTTGACCTCTACCA	CTGAAACACGTCTGGCAGAT	50.0	50.0	58.0	58.2	1650 bp	0.5	58.3
atpD_p2	CCCATAAGTACAGCCCCAAC	CTGAAACACGTCTGGCAGAT	55.0	50.0	57.9	58.2	1384 bp	0.5	58.0
atpD_p3	ACTCACGTTGACCTCTACCA	TGAAACACGTCTGGCAGATT	50.0	45.0	58.0	57.7	1649 bp	0.5	57.9
atpD_p4	ACTCACGTTGACCTCTACCA	TCGGTTTTACGTAATCGCGA	50.0	45.0	58.0	57.7	1471 bp	0.5	57.9
atpD_p5	TGCGATCCCATCTCCTACTT	CTGAAACACGTCTGGCAGAT	50.0	50.0	57.9	58.2	1271 bp	0.5	57.7