Hotspot

atpA

Genome · 33437-34942 · 1506 bp

Back to Chorda

Pi 0.296
MarkerSeek score 81.10
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
Genome
length_bp
1506
variable_sites
435
pi
0.296
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 GGGCTAGCTGTTATGGAACC AGGGCCTGAGTTTACAACTTC 55.0 47.6 58.0 57.9 2316 bp 0.5 50.6
atpA_p2 GGGCTAGCTGTTATGGAACC GGGCCTGAGTTTACAACTTCT 55.0 47.6 58.0 57.9 2315 bp 0.5 50.6
atpA_p3 ATTATGGTGAGCCTGCTTGG AGGGCCTGAGTTTACAACTTC 50.0 47.6 57.9 57.9 2396 bp 0.5 50.4
atpA_p4 ATTATGGTGAGCCTGCTTGG GGGCCTGAGTTTACAACTTCT 50.0 47.6 57.9 57.9 2395 bp 0.5 50.4
atpA_p5 GGAACCTTCTGCCTTAGGTG GGGCCTGAGTTTACAACTTCT 55.0 47.6 57.9 57.9 2301 bp 0.5 49.9