Hotspot

trnV-UAC-trnT-UGU

Genome · 125323-125453 · 131 bp

Back to Chorda

Pi 0.366
MarkerSeek score 72.60
Primer available yes
Label trnV-UAC-trnT-UGU

Feature Metadata

label_name
trnV-UAC-trnT-UGU
feature_type
igs
region
Genome
length_bp
131
variable_sites
48
pi
0.366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnT-UGU_p1 CTACCACTGAGCTAACTGCC GTTAAGGCAGTGGGTTGTGA 55.0 50.0 57.7 58.0 362 bp 0.5 57.4
trnV-UAC-trnT-UGU_p2 CTACCACTGAGCTAACTGCC AAGTCCCGTCATTCACCCTA 55.0 50.0 57.7 58.1 323 bp 0.5 57.2
trnV-UAC-trnT-UGU_p3 CTACCACTGAGCTAACTGCC AGTCCCGTCATTCACCCTAA 55.0 50.0 57.7 58.1 322 bp 0.5 57.2
trnV-UAC-trnT-UGU_p4 CTACCACTGAGCTAACTGCC GGTTCAAGTCCCGTCATTCA 55.0 50.0 57.7 57.8 328 bp 0.5 56.2
trnV-UAC-trnT-UGU_p5 CTACCACTGAGCTAACTGCC TTCAAGTCCCGTCATTCACC 55.0 50.0 57.7 57.8 326 bp 0.5 56.2