Hotspot

psbD-trnN-GUU

Genome · 121767-122003 · 237 bp

Back to Chorda

Pi 0.489
MarkerSeek score 72.50
Primer available yes
Label psbD-trnN-GUU

Feature Metadata

label_name
psbD-trnN-GUU
feature_type
igs
region
Genome
length_bp
237
variable_sites
116
pi
0.489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnN-GUU_p1 GGTCAAATAACCCGCTACGT TTGAAATTGCGGGAGTGGAT 50.0 45.0 58.0 57.8 965 bp 0.5 58.2
psbD-trnN-GUU_p2 GGTCAAATAACCCGCTACGT CTCAGTGGTAGAGCAATCGG 50.0 55.0 58.0 57.8 343 bp 0.5 58.2
psbD-trnN-GUU_p3 TATCGCAGCTACCCCAAAAC TTGAAATTGCGGGAGTGGAT 50.0 45.0 58.0 57.8 1450 bp 0.5 58.0
psbD-trnN-GUU_p4 TATCGCAGCTACCCCAAAAC CTCAGTGGTAGAGCAATCGG 50.0 55.0 58.0 57.8 828 bp 0.5 58.0
psbD-trnN-GUU_p5 TGAGCTTCTGGTCCCCATAA TTGAAATTGCGGGAGTGGAT 50.0 45.0 58.0 57.8 1209 bp 0.5 58.0