Hotspot

rnl-trnA-UGC

Genome · 27981-28036 · 56 bp

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Pi 0.381
MarkerSeek score 85.80
Primer available yes
Label rnl-trnA-UGC

Feature Metadata

label_name
rnl-trnA-UGC
feature_type
igs
region
Genome
length_bp
56
variable_sites
16
pi
0.381
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rnl-trnA-UGC_p1 CCCCCGTAAGCCCTTATTTC CCATCTGGGGGTATAGCTCA 55.0 55.0 58.0 58.0 161 bp 0.5 59.6
rnl-trnA-UGC_p2 CCCCGTAAGCCCTTATTTCC CCATCTGGGGGTATAGCTCA 55.0 55.0 58.0 58.0 160 bp 0.5 59.6
rnl-trnA-UGC_p3 TCGTAGCTTGTTTCCAGCTC CCATCTGGGGGTATAGCTCA 50.0 55.0 57.9 58.0 236 bp 0.5 59.1
rnl-trnA-UGC_p4 CTCGTAGCTTGTTTCCAGCT CCATCTGGGGGTATAGCTCA 50.0 55.0 57.9 58.0 237 bp 0.5 59.1
rnl-trnA-UGC_p5 ATATCTCCGGCTCGTAGCTT CCATCTGGGGGTATAGCTCA 50.0 55.0 58.1 58.0 247 bp 0.5 59.1