Hotspot

rpoA

LSC · 79663-80670 · 1008 bp

Back to Chonemorpha

Pi 0.00397

MarkerSeek score 61.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 4
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATTGTACTAGGGACTCA	AATGGCTGTAGACCTCCGAA	40.0	50.0	52.7	58.4	1200 bp	1.0	40.3
rpoA_p2	TGAATTGTACTAGGGACTCA	ATGGCTGTAGACCTCCGAAA	40.0	50.0	52.7	58.4	1199 bp	1.0	40.3
rpoA_p3	TGAATTGTACTAGGGACTCA	TAATGGCTGTAGACCTCCGA	40.0	50.0	52.7	57.6	1201 bp	1.0	40.3
rpoA_p4	TGAATTGTACTAGGGACTCA	GGCTGTAGACCTCCGAAAAA	40.0	50.0	52.7	57.5	1197 bp	1.0	40.3
rpoA_p5	TGAATTGTACTAGGGACTCAA	ATGGCTGTAGACCTCCGAAA	38.1	50.0	53.6	58.4	1199 bp	1.0	40.3